Reproductive Sciences

List of transgenic services

Rapid colony expansion

species: Mice and rats
duration: 12 weeks
deliverable:
Restitution of the produced SOPF colony, according to your needs
 

Description of our service

Rapid colony expansion means producing in a single step as many animals as wanted carrying the genetic modification of the strain. This is made possible through the use of in vitrofertilisation using a male from the strain and wild type female eggs from donors. Since the number of females is not limited, it is possible to obtain, in a mastered quantity, a batch of homogeneous animals as regards age, sex and genotype. The animals obtained will be SOPF ensuring a simultaneous rederivation.

The rapid colony expansion provides animals quickly and directly available for experimentation or to start a colony (the use of female wild types implicates a first generation of heterozygous for the transgene). The rapid colony expansion is also useful for lines with early mortality of individuals (e.g., neuromuscular disease).

Our method

  • Receipt of your animals or sperm straws in one of our external laboratories to enable the containment of contaminated strains.
  • in vitro fertilisation (oocytes obtained from superovulated females from JANVIER LABS strain, choice of donor strains).
  • Culture of embryos to 2-cell stage.
  • Successive washes of the embryos with sterile medium.
  • Transfer of washed embryos to recipient "pseudopregnant" SOPF females.
  • Transfer of recipient females to an isolator.
  • Birth of pups.
  • Health monitoring programme tests to confirm SOPF status.
  • Shipment of SOPF animals according to your needs.

Possible options

  • Straws available in our storage tanks.
  • Tail biopsies and identification of pups from your strain: depending on the genotype of the original animals, it may be necessary to make a genotyping of decontaminated animals.
  • Cryopreservation and recovery of strains using sperm or embryos to decontaminate and cryopreserve your strain at the same time.
  • Continued breeding your transgenic strains in isolators.

Our added value

  • 50 years of experience in the management, breeding and production of rodents.
  • The organisation of the transport and receipt of your biological material from anywhere in the world.
  • The shipment of the recovered and decontaminated animals thanks to our dedicated and internal transport service.
  • The monitoring of your project and the transmission of regular reporting by your dedicated project manager.
  • The introduction of "sanitary barriers" with equipment and dedicated staff ensures a controlled and optimal decontamination of your strains.
  • A team of experts to advise you on the ideal mating scheme, the requested quantity of animals, the strain of oocytes donor females, the need of a specific genotyping.
  • Health monitoring programme tests performed by independent accredited organisations.

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