Reproductive Sciences

List of transgenic services
species: Mice and rats
duration: 3-5 months
deliverable:
Restitution of the SOPF animals without any guarantee of results
 

Description of our service

Rescuing of a strain may be necessary when one is faced with fertility problems, production stopping or incidents in the facilities. The use of this service can also be a solution if available animals are too old.

At JANVIER LABS's, we master various advanced methods that will allow us to diagnose the problem. Depending on the animals that you have available, we can offer you the solution to rescue your strain.

If you have only males: it is often possible to recover viable animals after in vitro fertilisation. This method will allow you to quickly get started with a new colony.

If you have only females: we can offer you an ovary transplant to a co-isogenic genetic background.

Our method

Receipt of your animals in one of our external laboratories to enable the containment of contaminated strains.

  • to rescue the strain using males:​
    - preliminary tests for detection of cryptorchidism and mating with JANVIER LABS's females(detection of vaginal plugs).
    - additional test by in vitro fertilisation (oocytes obtained from superovulated JANVIER LABS females).
    - embryo transfer and transfer to the isolators.
    - birth of pups from your strain.
    - health monitoring programme tests for validation of SOPF status.
    - shipping of SOPF animals.
     
  • to rescue the strain using females:
    - preliminary tests on the female by mating with a fertile JANVIER LABS male (detection of vaginal plugs).
    - additional tests by ovariectomy and transplantation of ovarian tissue to JANVIER LABS females with the same genetic background as the strain of interest.
    - offspring matin.
    - embryo transfer and transfer to the isolators.
    - birth of pups from your strain.
    - health monitoring programme tests for validation of SOPF status.

Possible options

  • Tail biopsies and identification of pups from your strain: depending on the genotype of the original animals, it may be necessary to make a genotyping of decontaminated animals.
  • Cryopreservation and recovery of strains using sperm or embryos to decontaminate and cryopreserve your strain at the same time.
  • Breeding your transgenic strains in isolators.

Our added value

  • 50 years of experience in the management, breeding and production of rodents.
  • The organisation of the transport and receipt of your biological material from anywhere in the world.
  • The shipment of the recovered and decontaminated animals thanks to our dedicated and internal transport service.
  • The monitoring of your project and the transmission of regular reporting by your dedicated project manager.
  • The introduction of "sanitary barriers" with equipment and dedicated staff ensures a controlled and optimal decontamination of your strains.
  • A team of experts to advise you on the ideal mating scheme, the requested quantity of animals, the strain of oocytes donor females, the need of a specific genotyping.
  • Health monitoring programme tests performed by independent accredited organisations.

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