Reproductive Sciences

List of transgenic services

Embryo cryopreservation

species: Mice and rats
duration: 8 to 12 weeks
pre-requisite:
5 males aged 10-20 weeks or 5 to 10 males aged 10 - 20 weeks and 25 to 30 females aged less than 20 weeks
deliverable:
150-200 embryos frozen with your males and females - or - 250 embryos frozen with your males and females from JANVIER LABS in vitro quality control included
 

Description of our service

Cryopreservation is an ideal solution to provide a safety stock of a strain in case of a problem on your facilities or to preserve a strain that is rarely used.

The cryopreservation of embryos is generally recommended for complex strains and where preservation of the genetic background is required. Indeed, the cryopreserved genetic material will be fully recovered and this technique will freeze the characteristics and prevent genetic drift.

Our method

  • Receipt of your animals in one of our external laboratories to enable the containment of contaminated strains.
  • Mating schemes to choose from:
    - your males and your females
    - your males with our females (or vice versa).
  • Collection of embryos and successive washes of the embryos with sterile medium.
  • Freezing of embryos by vitrification (2-cell stage in 10-20 straws).
  • in vitro quality control QC1: by thawing and culture of embryos for 24 hours. Expressed in % of embryos developing to 4-8-cell stage.
  • Cryopreservation in liquid nitrogen.

Possible options

  • in vivo quality control, QC2: thawed embryos reimplanted into pseudopregnant females. Birth rate check.
  • Dual cryo-storage at JANVIER LABS: per year cryo-storage.
  • Return of straws containing frozen embryos from your strain.
  • Recovery of embryos (decontaminated animals).
  • Breeding your transgenic strains in isolators.
  • Cryopreservation of sperm and recovery of the strain.
  • Combination of rederivation and cryopreservation for the recovery of the strain from cryopreserved embryos.

Our added value

  • 50 years of experience in the management, breeding and production of rodents.
  • The organisation of the transport and receipt of your biological material from anywhere in the world.
  • The monitoring of your project and the transmission of regular reporting by your dedicated project manager.
  • A team of experts to advise you on the choice of cryopreservation method depending on the characteristics of your strain, the ideal mating scheme, the requested quantity of animals, the quality controls.

Request for specific quote on this product

clic here for a specific quote on this product

Go back