List of transgenic services
species: Mice and rats
duration: 8 to 12 weeks
5 males aged 10-20 weeks or 5 to 10 males aged 10 - 20 weeks and 25 to 30 females aged less than 20 weeks
150-200 embryos frozen with your males and females - or - 250 embryos frozen with your males and females from JANVIER LABS in vitro quality control included
Description of our service
Cryopreservation is an ideal solution to provide a safety stock of a strain in case of a problem on your facilities or to preserve a strain that is rarely used.
The cryopreservation of embryos is generally recommended for complex strains and where preservation of the genetic background is required. Indeed, the cryopreserved genetic material will be fully recovered and this technique will freeze the characteristics and prevent genetic drift.
Receipt of your animals in one of our external laboratories to enable the containment of contaminated strains.
Mating schemes to choose from:
- your males and your females
- your males with our females (or vice versa).
Collection of embryos and successive washes of the embryos with sterile medium.
Freezing of embryos by vitrification (2-cell stage in 10-20 straws).
in vitro quality control QC1: by thawing and culture of embryos for 24 hours. Expressed in % of embryos developing to 4-8-cell stage.
- Cryopreservation in liquid nitrogen.
Our added value
50 years of experience in the management, breeding and production of rodents.
The organisation of the transport and receipt of your biological material from anywhere in the world.
The monitoring of your project and the transmission of regular reporting by your dedicated project manager.
A team of experts to advise you on the choice of cryopreservation method depending on the characteristics of your strain, the ideal mating scheme, the requested quantity of animals, the quality controls.