Rapid colony expansion
Rapid colony expansion means producing in a single step as many animals as wanted carrying the genetic modification of the strain.
This is made possible through the use of in vitro fertilisation using a male from the strain and wild type female eggs from donors.
Since the number of females is not limited, it is possible to obtain, in a mastered quantity, a batch of homogeneous animals as regards age, sex and genotype. The animals obtained will be SOPF ensuring a simultaneous rederivation.
The rapid colony expansion provides animals quickly and directly available for experimentation or to start a colony (the use of female wild types implicates a first generation of heterozygous for the transgene).
The rapid colony expansion is also useful for lines with early mortality of individuals (e.g., neuromuscular disease).
Rapid colony expansion allows to obtain large animal batches directly available very quickly for an experiment through the use of IVF on a large scale.
Added values of JANVIER LABS:
In a real commitment to transparency, all analyses carried out on our animals are performed by external reference laboratories: BioDoc or IDEXX BioResearch Europe in Germany, QM Diagnostics in the Netherlands or LDA 53 in France.
These laboratories are:
• independent, signifying the neutrality of the results,
• recognized, signifying the objectivity of the results,
• and accredited or validated by accreditation organisations (COFRAC for France, DAkkS for Germany or CCKL for the Netherlands), signifying the reliability of the methods used.
The reliability of analysis methods used is also part of the essential component for the consistency of the results. The external laboratories we entrust to carry out the analysis of all our animals, use standardized and validated analysis methods according to international references.
Receipt of your animals or sperm straws from your strain.
In Vitro Fertilisation.
Culture of embryos to 2-cell stage.
Successive washes of the embryos with sterile medium.
Transfer of washed embryos to recipient “pseudopregnant" SOPF females.
Transfer of recipient females to an isolator.
Birth of pups.
Health monitoring programme tests to confirm SOPF status.
Shipment of SOPF animals according to your needs or transfer of the colony to customized breeding.