Rederivation by embryo transfer
The rederivation (or decontamination) by embryo transfer allows you to obtain SOPF (free of pathogens and some opportunistic microorganisms) animals from infected animals.
Decontamination may be necessary to remove the influence of infectious agents on an experiment. It is also necessary to securely exchange a line with a colleague based on the health status of the respective animal facilities.
Added values of JANVIER LABS:
The organisation of the transport and receipt of your biological material from anywhere in the world!
- Possibility of rederivation by rapid embryo transfer: delivery of pregnant females.
- Possibility to perform it by IVF if too few animals available.
- The shipment of decontaminated animals thanks to our dedicated and internal transport service.
- The monitoring of your project and the sending of regular reporting by your dedicated project manager.
- The introduction of “health barriers” with equipment and dedicated staff ensures a controlled and optimal decontamination of your strains.
A team of experts to advise you on:
- the ideal mating scheme,
- the requested quantity of animals,
- the need of genotyping.
Health monitoring programme tests performed by independent accredited laboratories
Collection of the animals necessary to the service, by our transport service when it is located in our transport zone or by our approved partner providers
Receipt of your animals at our External Reproductive Sciences Laboratory
Sampling and storage of biopsies for potential genotype control
Natural mating of your males with our females or with JANVIER LABS WT females of the corresponding genetic background or of another background of your choice
Collection of embryos and successive washes of the embryos with sterile medium
Transfer of washed embryos to recipient “pseudopregnant” SOPF females
Housing of reimplanted females in isolators
Transmission of regular reports at each stage during the follow-up of the project
Electronic identification of the SOPF animals obtained from the strain
Sampling of tail biopsies (or ear biopsies if you prefer)
Creation of a genotyping file (number of biopsy and identification number of the animal)
Shipment of the biopsies and shipment of the genotyping file to the customer
Receipt of the genotyping results and of the selection of animals of interest made by the customer before shipment
Supply of a chip reader along with the delivery of SOPF animals with the health certificate
A complete SOPF health control
Shipment of SOPF animals according to your needs or transfer of the colony to customized breeding
Keeping the remainder of your original animals 15 days after shipment of the healthy animals