Speed Congenics - Backcross
Transferring a mutation from a genetic background to another.
The transmission of a mutation can be tested at each generation by measuring the % of genetic markers of a recipient strain (a panel of 2050 SNPs (mice) or of 740 SNPs (rats) is analysed and covers the whole autosomes with an average interval of 5Mb). The 2 highest results are used for the next generation.
Using this technique, over 5 generations, animals consist of more than 99.9% of the recipient genome.
The aim of “speed congenics” or backcross is to significantly accelerate the required time to transfer a mutation of interest of a genetic background (donor genome) to another genetic background (recipient genome). The time is reduced in general by 50% compared to classic backcross carried out over 10 generations.
- TIME GAIN: 50% time reduction compared to classic backcross. Over 5 generations, animals consist of more than 99.9% of the recipient genome.
- SNPs ANALYSIS: analysis at each generation from a panel of 2050 SNPs (mice) or of 740 SNPs (rats) over about 20 samples.
- PURE GENETIC BACKGROUND: reduction of the variability of the phenotype due to the genetic background.
Added values of JANVIER LABS:
Analysis from a panel of 2050 SNPs (mice) or of 740 SNPs (rats). In order to have the most representative results, it is recommended to analyse 15-20 animals at each generation.